thr77 for Dummies
thr77 for Dummies
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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has long been observed in many tumors, which leads to chromosome instability, and so may possibly contribute to tumorigenesis. This protein was discovered to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in mobile-cycle controlled histone gene expression and plays a essential role in endorsing cell-cycle development from the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are already described. Two more splice variants have been reported but specific nucleotide sequence details is not still obtainable.
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Blocking peptides are peptides that bind particularly on the concentrate on antibody and block antibody binding. These peptide typically incorporates the epitope recognized with the antibody.
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This guidebook outlines additional possible optimization of mobile and immunoassay parameters to be sure the best possible results are...
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This guideline outlines further possible optimization of cellular and immunoassay parameters to ensure the best possible results are...
Antibodies bound to the blocking peptide no longer bind to the epitope on the concentrate on protein. By evaluating the staining with the blocked antibody as opposed to the antibody by yourself, one can see which staining is particular.
Target Information Cyclin E more info belongs to the really conserved cyclin spouse and children, whose customers are characterized by a dramatic periodicity in protein abundance through the mobile cycle. Cyclins purpose as regulators of CDK kinases. Diverse cyclins exhibit distinctive expression and degradation designs which contribute into the temporal coordination of each and every mitotic function. Cyclin E forms a posh with and features as being a regulatory subunit of CDK2, whose exercise is needed for mobile cycle G1/S changeover. This protein accumulates for the G1-S stage boundary and is degraded as cells development via S section.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by the two antibodies...
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